General Interview Questions - RNA
1. What are the functions of RNA? Why do you need it?
2. What is RNA made of?
3. What is the structure of RNA?
4. What are the types of RNA?
5. How is RNA modified/regulated?
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1. To be used by the cell, DNA is transcribed into ribonucleic acid (RNA).
2. RNA is synthesized by a DNA-dependent RNA polymerase using ribonucleotides.
3. A ribonucleotide consists of a phosphate group, attached to the 5’ carbon of the sugar ribose. One of four nitrogenous ‘bases’, either a pyrimidine (cytosine or uracil) or a purine (guanine or adenine), is attached to the 1’ carbon of the sugar.
4. RNAs can perform many functions: structural, catalytic, informational and regulative. Translation involves mRNA, tRNA and the RNAs of the ribosome.
5. The enzymes that mediate RNA synthesis can synthesize RNA de novo, that is without a primer.
6. After their synthesis (transcription), RNA can be modified in various ways, for example by splicing, 5' inverted G cap addition, RNA editing and post-transcriptional modification of the nucleotide bases.
7. Given their ability to both template their own replication and to act as catalysts, RNAs are often assumed to have played a key roll in the origins of life. This is so-called RNA world hypothesis.
8. A ribosomal RNA catalyses peptide bond formation during mRNA/tRNA-based translation on ribosomes.

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