General Interview Questions - RNA 1. What are the functions of RNA? Why do you need it? 2. What is RNA made of? 3. What is the structure of RNA? 4. What are the types of RNA? 5. How is RNA modified/regulated?

Concept Statements Emphasis in your teaching General importance 1. To be used by the cell, DNA is transcribed into ribonucleic acid (RNA). Emphasized Mentioned Not Covered Irrelevant Critical Important Marginal Wrong 2. RNA is synthesized by a DNA-dependent RNA polymerase using ribonucleotides. Emphasized Mentioned Not Covered Irrelevant Critical Important Marginal Wrong 3. A ribonucleotide consists of a phosphate group, attached to the 5’ carbon of the sugar ribose. One of four nitrogenous ‘bases’, either a pyrimidine (cytosine or uracil) or a purine (guanine or adenine), is attached to the 1’ carbon of the sugar. Emphasized Mentioned Not Covered Irrelevant Critical Important Marginal Wrong 4. RNAs can perform many functions: structural, catalytic, informational and regulative. Translation involves mRNA, tRNA and the RNAs of the ribosome. Emphasized Mentioned Not Covered Irrelevant Critical Important Marginal Wrong 5. The enzymes that mediate RNA synthesis can synthesize RNA de novo, that is without a primer. Emphasized Mentioned Not Covered Irrelevant Critical Important Marginal Wrong 6. After their synthesis (transcription), RNA can be modified in various ways, for example by splicing, 5' inverted G cap addition, RNA editing and post-transcriptional modification of the nucleotide bases. Emphasized Mentioned Not Covered Irrelevant Critical Important Marginal Wrong 7. Given their ability to both template their own replication and to act as catalysts, RNAs are often assumed to have played a key roll in the origins of life. This is so-called RNA world hypothesis. Emphasized Mentioned Not Covered Irrelevant Critical Important Marginal Wrong 8. A ribosomal RNA catalyses peptide bond formation during mRNA/tRNA-based translation on ribosomes. Emphasized Mentioned Not Covered Irrelevant Critical Important Marginal Wrong

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