Measuring bacteriophage burst size |
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Does each phage-infected
bacterial cell release the same number of phage or does the number
vary from cell to cell? If it varies, can we define the
factors involved in this variation? In a classic experiment, Max Delbrück and Salvador Luria measured the number of virions released from individual bacteria infected with bacteriophage alpha |
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They found that the burst size number varied dramatically from cell to cell. Our question is, does T4 display the same behavior? To determine the average number of virions produced per T4 infected E. coli cell we will follow the same protocol used by Luria and Delbrück. |
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Lab report part 3
Question to answer: In the first part of experiment its critical to dilute the phage + bacteria mixture quickly. After diluting there is no particular urgency in plating out the samples. Explain why this is the case. |
Use Wikipedia |
revised
20-Apr-2006
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